Reversed‐phase vortex‐assisted liquid–liquid microextraction: A new sample preparation method for the determination of amygdalin in oil and kernel samples |
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| Authors: | Mohammad Hosseini Rouhollah Heydari Mohammad Alimoradi |
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| Affiliation: | 1. Department of Chemistry, Faculty of Sciences, Islamic Azad University, Arak Branch, Arak, Iran;2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran |
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| Abstract: | A novel, simple, and rapid reversed‐phase vortex‐assisted liquid–liquid microextraction coupled with high‐performance liquid chromatography has been introduced for the extraction, clean‐up, and preconcentration of amygdalin in oil and kernel samples. In this technique, deionized water was used as the extracting solvent. Unlike the reversed‐phase dispersive liquid–liquid microextraction, dispersive solvent was eliminated in the proposed method. Various parameters that affected the extraction efficiency, such as extracting solvent volume and its pH, vortex, and centrifuging times were evaluated and optimized. The calibration curve shows good linearity (r2 = 0.9955) and precision (RSD < 5.2%) in the range of 0.07–20 μg/mL. The limit of detection and limit of quantitation were 0.02 and 0.07 μg/mL, respectively. The recoveries were in the range of 96.0–102.0% with relative standard deviation values ranging from 4.0 to 5.1%. Unlike the conventional extraction methods for plant extracts, no evaporative and re‐solubilizing operations were needed in the proposed technique. |
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| Keywords: | Amygdalin High‐performance liquid chromatography Liquid– liquid microextraction Reversed phase Sample preparation |
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