Preparation of 17β‐estradiol‐imprinted material by surface‐initiated atom transfer radical polymerization and its application |
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| Authors: | Yanru Gong Yuling Niu Xiaohan Gong Meihua Ma Xiaowei Ren Weihua Zhu Ruiming Luo Bolin Gong |
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| Affiliation: | 1. School of Chemistry and Chemical Engineering, Beifang University of Nationalities, Yinchuan, China;2. Key Laboratory of Energy and Chemical Engineering, Ningxia University, China;3. School of Chemistry and Chemical Engineering of Jiangsu University, China;4. Agricultural College, Ningxia University, China |
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| Abstract: | A novel 17β‐estradiol molecularly imprinted polymer was grafted onto the surface of initiator‐immobilized silica by surface‐initiated atom transfer radical polymerization. The resulting molecularly imprinted polymer was characterized by elemental analysis and thermogravimetric analysis. The binding property of molecularly imprinted polymer for 17β‐estradiol was also studied with both static and dynamic methods. The results showed that the molecularly imprinted polymer possessed excellent recognition capacity for 17β‐estradiol (180.65 mg/g at 298 K), and also exhibited outstanding selectivity for 17β‐estradiol over the other competitive compounds (such as testosterone and progesterone). Then, the determination of trace 17β‐estradiol in beef samples was successfully developed by using molecularly imprinted polymer solid‐phase extraction coupled with high‐performance liquid chromatography. The limit of detection was 0.25 ng/mL, and the amount of 17β‐estradiol in beef samples was detected at 2.83 ng/g. This work proposed a sensitive, rapid, reliable, and convenient approach for the determination of trace 17β‐estradiol in complicated beef samples. |
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| Keywords: | Atom transfer radical polymerization Beef samples 17β ‐Estradiol Molecularly imprinted polymers Solid‐phase extraction |
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