Antioxidative vs cytotoxic activities of organotin complexes bearing 2,6‐di‐tert‐butylphenol moieties |
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| Authors: | TA Antonenko DB Shpakovsky MA Vorobyov YuA Gracheva EV Kharitonashvili LG Dubova EF Shevtsova VA Tafeenko LA Aslanov AG Iksanova YuG Shtyrlin ER Milaeva |
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| Affiliation: | 1. Department of Medicinal Chemistry and Fine Organic Synthesis, Lomonosov Moscow State University, Moscow, Russia;2. Institute of Physiologically Active Compounds of Russian Academy of Sciences, Chernogolovka, Russia;3. Kazan (Volga Region) Federal University, Kazan, Russia |
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| Abstract: | Two series of organotin(IV) complexes with Sn–S bonds on the base of 2,6‐di‐tert‐butyl‐4‐mercaptophenol ( L 1 SH ) of formulae Me2Sn(L1S)2 ( 1 ); Et2Sn(L1S)2 ( 2 ); Bu2Sn(L1S)2 ( 3 ); Ph 2 Sn(L1S)2 ( 4 ); (L1)2Sn(L1S)2 ( 5 ); Me3Sn(L1S) ( 6 ); Ph3Sn(L1S) ( 7 ) (L1 = 3,5‐di‐tert‐butyl‐4‐hydroxyphenyl), together with the new ones Me3SnCl(L2)] ( 8 ), Me2SnCl2(L2)2] ( 9 ) ( L 2 = 2‐(N‐3′,5′‐di‐tert‐butyl‐4′‐hydroxyphenyl)‐iminomethylphenol) were used to study their antioxidant and cytotoxic activity. Novel complexes 8 , 9 of MenSnCl4 ? n (n = 3, 2) with Schiff base were synthesized and characterized by 1H, 13C NMR, IR and elemental analysis. The crystal structures of compounds 8 and 9 were determined by X‐ray diffraction analysis. The distorted tetrahedral geometry around the Sn center in the monocrystals of 8 was revealed, the Schiff base is coordinated to the tin(IV) atom by electrostatic interaction and formation of short contact Sn–O 2.805 Å. In the case of complex 9 the distorted octahedron coordination of Sn atom is formed. The antioxidant activity of compounds as radical scavengers and reducing agents was proved spectrophotometrically in tests with stable radical DPPH, reduction of Cu2+ (CUPRAC method) and interaction with superoxide radical‐anion. Moreover, compounds have been screened for in vitro cytotoxicity on eight human cancer cell lines. A high activity against all cell lines with IC50 values 60–160 nM was determined for the triphenyltin complex 7 , while the introduction of Schiff base decreased the cytotoxicity of the complexes. The influence on mitochondrial potential and mitochondrial permeability for the compounds 8 and 9 has been studied. It is shown that studied complexes depolarize the mitochondria but don't influence the calcium‐induced mitochondrial permeability transition. |
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| Keywords: | 2 6‐di‐tert‐butylphenol antioxidants cytotoxicity organotin(IV) complexes Schiff bases |
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