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等度反相高效液相色谱法测定茶多酚中的儿茶素和咖啡因
引用本文:唐根源,吴红京,吴棱,李兆基,姚元根.等度反相高效液相色谱法测定茶多酚中的儿茶素和咖啡因[J].色谱,2001,19(3):233-236.
作者姓名:唐根源  吴红京  吴棱  李兆基  姚元根
作者单位:中国科学院福建物质结构研究所
基金项目:福建省自然科学基金资助项目(B9810012)
摘    要: 介绍一种简便的等度反相高效液相色谱分析茶多酚中 5种儿茶素和咖啡因的快速方法。样品总的分析时间在 0 5h内。色谱柱为ResolveC18;流动相为水 体积分数为 85 %的磷酸水溶液 乙腈 N ,N 二甲基甲酰胺 (DMF)(体积比为 85 9∶1∶12 0∶2 0 ) ;柱温为 43℃ ;紫外检测波长为 2 80nm。研究了流动相中改良剂DMF与容量因子的关系。被测组分的含量与其峰面积有良好的线性关系 (r =0 .9992~ 0 .9999) ;加标回收率在 83.33%~ 10 4.42 %(RSD在 0 .74%~ 1.43% )。

关 键 词:等度高效液相色谱法  儿茶素  咖啡因  茶多酚
文章编号:1000-8713(2001)03-0233-03
修稿时间:2000年10月13

Separation of Catechins and Caffeine in Tea Polyphenol byIsocratic Elution High Performance Liquid Chromatography
TANG Gen yuan,WU Hong jing,WU Ling,LI Zhao ji,YAO Yuan gen.Separation of Catechins and Caffeine in Tea Polyphenol byIsocratic Elution High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2001,19(3):233-236.
Authors:TANG Gen yuan  WU Hong jing  WU Ling  LI Zhao ji  YAO Yuan gen
Affiliation:State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
Abstract:The catechins, particularly in green tea, have been found to possess anti-mutagenic and anti-tumorigenic properties. As each catechin possesses distinct properties, a simple and rapid method that could be used for analysis of individual catechins in a complex mixture would be necessary. A relatively simple and rapid method for simultaneous separation of five catechins and caffeine in tea polyphenol by isocratic elution high performance liquid chromatography has been developed. The analysis could be finished within 30 min. They were measured using Resolve C18 column (at 43 degrees C) and UV detector (at 280 nm), water-85% phosphoric acid aqueous solution-acetonitrile-dimethyl formamide(DMF) (859:1:120:20, V/V) as mobile phase. There was a good linear relationship between the content of component and its peak area for catechins and caffeine, with the correlation coefficients of 0.9992-0.9999. The average recoveries (n = 5) were 83.33%-104.42%, and the relative standard deviations (n = 6) were 0.74%-1.43%. The effect of concentration of DMF in mobile phase was studied.
Keywords:isocratic elution high performance liquid chromatography  catechin  caffeine  tea polyphenol
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