| 超高效液相色谱-电喷雾串联四极杆质谱法检测果汁和葡萄酒中的27种工业染料 |
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| 引用本文： | 赵珊,张晶,杨奕,邵兵.超高效液相色谱-电喷雾串联四极杆质谱法检测果汁和葡萄酒中的27种工业染料[J].色谱,2010,28(4):356-362. |
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| 作者姓名： | 赵珊 张晶 杨奕 邵兵 |
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| 作者单位： | 北京市疾病预防控制中心, 北京 100013 |
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| 基金项目： | 北京市科技计划项目，卫生部公益性行业科研专项项目 |
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| 摘 要： | 建立了以超高效液相色谱-电喷雾串联四极杆质谱(UPLC-MS/MS)检测果汁和葡萄酒中27种工业染料的方法。样品经乙腈振荡提取,在盐析作用下分层,目标化合物转移至乙腈层中。目标化合物在梯度洗脱条件下经C18柱分离后采用多反应监测(MRM)模式进行检测。其中24种工业染料使用正离子模式检测,流动相为乙腈和0.1%的甲酸水溶液;其余3种工业染料则采用负离子模式检测,流动相为乙腈和水。结果表明: 果汁中27种工业染料的定量限(LOQ)为0.1～50 μg/kg,回收率为57.0%～117.7%,相对标准偏差为2.4%～17.7%。葡萄酒中的LOQ为0.2～50 μg/kg,回收率为40.8%～109.4%,相对标准偏差为1.6%～17.9%。该方法操作简单,灵敏度高,实现了不同种类的工业染料的同时提取,适合于软饮料中非法添加工业染料的快速检测。
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| 关 键 词： | 超高效液相色谱-电喷雾串联四极杆质谱 工业染料 果汁 葡萄酒 |
| 收稿时间： | 2009-9-28 |
| 修稿时间： | 2009-11-28 |
Determination of 27 industrial dyes in juice and wine using ultra performance liquid chromatography with electrospray ionization tandem quadrupole mass spectrometry |
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| ZHAO Shan,ZHANG Jing,YANG Yi,SHAO Bing.Determination of 27 industrial dyes in juice and wine using ultra performance liquid chromatography with electrospray ionization tandem quadrupole mass spectrometry[J].Chinese Journal of Chromatography,2010,28(4):356-362. |
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| Authors: | ZHAO Shan ZHANG Jing YANG Yi SHAO Bing |
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| Affiliation: | Beijing Center for Disease Prevention and Control, Beijing 100013, China |
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| Abstract: | A method for the determination of 27 industrial dyes in juice and wine has been developed using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Acetonitrile was used as extraction solvent,and sodium chloride was added to salt out the analytes from the samples.Chromatographic separation was performed on a C18 column with the gradient elution and the mass spectrometric acquisition was carried out under the mode of multiple reaction monitoring (MRM).Twenty-four of the 27 dyes were... |
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| Keywords: | ultra performance liquid chromatography-electrospray ionization tandem quadrupole mass spectrometry (UPLC-ESI-MS/MS) industrial dyes juice wine |
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