| A New Method for Determining Dipole Moment Orientation of Single Molecules |
| |
| 作者姓名： | 王琛 刘力 王桂英 徐至展 |
| |
| 作者单位： | ShanghaiInstituteofOpticsandFineMechanics,ChineseAcademyofSciences,Shanghai201800 |
| |
| 摘 要： | A new imaging method is proposed to determine the three-dimensional dipole moment orientation of single fluorophore. Far-field microscopy can provide orientational information projected in the sample plane, while total internal reflection fluorescence microscopy (TIRFM) can offer the knowledge perpendicular to the surface because longitudinal electric-field components can be generated in total internal reflection geometry. By comparing fluorescence intensities measured with far-field epi-fluorescence microscopy and TIRFM, the exact information of single-fluorescent-molecule orientation is extracted. Detailed analysis of the method is given with a numerical example.
|
| 关 键 词： | 单分子 偶极矩定位 反射荧光显微技术 电场 分子物理学 |
| 本文献已被 维普 等数据库收录！ |
|
