| Calibration of Membrane Viscosity of the Reconstituted Vesicles by Measurement of the Rotational DIffusion of Bacteriorhodopsin |
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| 作者姓名： | 王敖金 胡坤生 |
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| 作者单位： | InstituteofBiophysics,ChineseAcademyofSciences,Beijing100101 |
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| 摘 要： | Membrane viscosity of the reconstituted vesicles was calibrated by rotational diffusion of bacteriorhodopin(BR) in dimyristoylphosphatidylcholine(DMPC) and egg phosphatidylcholine(PC) vesicles.Rotational diffusion of BR in the vesicles was measured by flash-induced absorption anisotropy decay.BR was,for the first time ,reconstituted successfully into DMPC and egg PC vesicles,From the measurement of flash-induced absorption anisotropy decay of BR,the value of rotational diffusion coefficient D was obtained from each curve fitting by a global fitting procedure and ,in turn,membrane viscosity η was estimated from D.The results have shown that membrane viscosity is temperature-dependent,It was decreased as temperature increased,but a transition occurred in the region of the respective phase transition of DMPC and egg PC,respectively,The decrease of η was fast near the phase transition for DMPC and egg PC.Few effects of lipid/BR ratio and glycerol or sucrose in suspension medium on membrane viscosity were found.
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| 关 键 词： | 蛋白质 膜粘度 旋转扩散测量 |
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