Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice |
| |
| Authors: | Ren Zhao-Yu Xu Xiao-Ming Wang Shui-Cai Xin Yue-Yong He Jun-Fang and Hou Xun |
| |
| Affiliation: | Institute of Photonics & Photo-Technology, Northwest University, Xi'an 710068, China; Laboratory of Photosynthesis Research, Nanjing Agricultural University, Nanjing 210095, China; State Key Laboratory of Transience Optics Technology, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710068, China; Laboratory of Photosynthesis Basic Research, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China |
| |
| Abstract: | A high yielding rice variety mutant (Oryza sativa L., Zhenhui 249) with low chlorophyll b (Chl b) has been discovered in natural fields. It has a quality character controlled by a pair of recessive genes (nuclear gene). The partial loss of Chl b in content affects the efficiency of light harvest in a light harvest complex (LHC), thus producing the difference of the exciting energy transfer and the efficiency of photochemistry conversion between the mutant and wild-type rice in photosynthetic unit. The efficiency of utilizing light energy is higher in the mutant than that in the wild-type rice relatively. For further discussion of the above-mentioned difference and learning about the mechanism of the increase in the photochemical efficiency of the mutant, the pico-second resolution fluorescence spectrum measurement with delay-frame-scanning single photon counting technique is adopted. Thylakoid membranes of the mutant and the wild-type rice are excited by an Ar^+ laser with a pulse width of 120 ps, repetition rate of 4 MHz and wavelength of 514 nm. Compared with the time and spectrum property of exciting fluorescence, conclusions of those ultrafast dynamic experiments are: 1) The speeds of the exciting energy transferred in photo-system I are faster than that in photo-system II in both samples. 2) The speeds of the exciting energy transfer of mutant sample are faster than those of the wild-type. This might be one of the major reasons why the efficiency of photosynthesis is higher in mutant than that in the wild-type rice. |
| |
| Keywords: | rice photosynthesis ultrafast process time constant exciting energy transfer |
|
| 点击此处可从《中国物理 B》浏览原始摘要信息 |
|
点击此处可从《中国物理 B》下载免费的PDF全文 |
|
