Protein crystallography beamline (PX‐BL21) at Indus‐2 synchrotron |
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| Authors: | Ashwani Kumar Biplab Ghosh H K Poswal K K Pandey Jagannath M V Hosur Abhilash Dwivedi Ravindra D Makde Surinder M Sharma |
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| Affiliation: | 1. High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India;2. Technical Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India;3. Advanced Centre for Treatment, Research and Education in Cancer, Navi Mumbai, India |
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| Abstract: | The protein crystallography beamline (PX‐BL21), installed at the 1.5 T bending‐magnet port at the Indian synchrotron (Indus‐2), is now available to users. The beamline can be used for X‐ray diffraction measurements on a single crystal of macromolecules such as proteins, nucleic acids and their complexes. PX‐BL21 has a working energy range of 5–20 keV for accessing the absorption edges of heavy elements commonly used for phasing. A double‐crystal monochromator Si(111) and Si(220)] and a pair of rhodium‐coated X‐ray mirrors are used for beam monochromatization and manipulation, respectively. This beamline is equipped with a single‐axis goniometer, Rayonix MX225 CCD detector, fluorescence detector, cryogenic sample cooler and automated sample changer. Additional user facilities include a workstation for on‐site data processing and a biochemistry laboratory for sample preparation. In this article the beamline, other facilities and some recent scientific results are briefly described. |
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| Keywords: | protein crystallography beamline bending magnet Indus‐2 |
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