Super‐Resolution Imaging of Plasma Membrane Glycans |
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| Authors: | Sebastian Letschert Dr Antonia Göhler Christian Franke Nadja Bertleff‐Zieschang Elisabeth Memmel Priv‐Doz?Dr Sören Doose Prof?Dr Jürgen Seibel Prof?Dr Markus Sauer |
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| Affiliation: | 1. Department of Biotechnology & Biophysics, Julius Maximilian University Wuerzburg, Am Hubland, 97074 Würzburg (Germany) http://www.super‐resolution.de;2. Institute of Organic Chemistry, Julius Maximilian University Wuerzburg, Am Hubland, 97074 Würzburg (Germany) |
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| Abstract: | Much of the physiology of cells is controlled by the spatial organization of the plasma membrane and the glycosylation patterns of its components, however, studying the distribution, size, and composition of these components remains challenging. A bioorthogonal chemical reporter strategy was used for the efficient and specific labeling of membrane‐associated glycoconjugates with modified monosaccharide precursors and organic fluorophores. Super‐resolution fluorescence imaging was used to visualize plasma membrane glycans with single‐molecule sensitivity. Our results demonstrate a homogeneous distribution of N‐acetylmannosamine (ManNAc)‐, N‐acetylgalactosamine (GalNAc)‐, and O‐linked N‐acetylglucosamine (O‐GlcNAc)‐modified plasma membrane proteins in different cell lines with densities of several million glycans on each cell surface. |
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| Keywords: | bioorthogonal chemistry click chemistry membrane glycans microscopy super‐resolution imaging |
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