Comprehensive metabolite profiling of Plantaginis Semen using ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry coupled with elevated energy technique |
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| Authors: | Dandan Wang Meng Qi Qiming Yang Renchao Tong Rui Wang S.W. Annie Bligh Li Yang Zhengtao Wang |
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| Affiliation: | 1. The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines and the STACM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China;2. Department of Life Sciences, Faculty of Science and Technology, University of Westminster, London, UK;3. Center for Chinese Medical Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine, Shanghai, China |
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| Abstract: | Plantaginis Semen is commonly used in traditional medicine to treat edema, hypertension, and diabetes. The commercially available Plantaginis Semen in China mainly comes from three species. To clarify the chemical composition and distinct different species of Plantaginis Semen, we established a metabolite profiling method based on ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry coupled with elevated energy technique. A total of 108 compounds, including phenylethanoid glycosides, flavonoids, guanidine derivatives, terpenoids, organic acids, and fatty acids, were identified from Plantago asiatica L., P. depressa Willd., and P. major L. Results showed significant differences in chemical components among the three species, particularly flavonoids. This study is the first to provide a comprehensive chemical profile of Plantaginis Semen, which could be involved into the quality control, medication guide, and developing new drug of Plantago seeds. |
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| Keywords: | Metabolite profiles Elevated energy mass spectrometry Plantaginis Semen Traditional Chinese medicines Ultra high performance liquid chromatography |
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