Two‐Photon Imaging of a Cellular Line Using Organic Fluorescent Nanoparticles Synthesized by Laser Ablation |
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| Authors: | Laura Aparicio‐Ixta J E Alba‐Rosales Gabriel Ramos‐Ortiz Mario Rodriguez Juan L Pichardo‐Molina Gerardo Gutierrez‐Juarez Myrna Sabanero‐Lopez Liss Flores Villavicencio Rosa Santillan Víctor M Tellez‐Lopez Daniel Martinez‐Fong |
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| Affiliation: | 1. Centro de Investigaciones en óptica, León, Gto., Mexico;2. División de Ciencias e Ingeniería, Campus León, Universidad de Guanajuato, León, Gto., Mexico;3. Departamento de Biología, Div. Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato, Gto., Mexico;4. Departamento de Química, Centro de Investigación y de Estudios, México, D.F., Mexico;5. Departamento de Fisiología, Biofísica y Neurociencias, México, D.F., Mexico;6. Departamento de Fisiología, Biofísica y Neurociencias, Programa de Doctorado en Nanociencias y Nanotecnología, México, D.F., Mexico |
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| Abstract: | A comparative study of the optical properties of organic fluorescent nanoparticles fabricated by laser ablation (NPs‐LA), reprecipitation (NPs‐RP), and microemulsion (NPs‐ME) methods is presented. These nanoparticles contain a fluorene‐based p‐conjugated molecule (BT2). Distinctive electronic transitions are observed in samples due to the specific way in which the molecule BT2 is assembled in each type of nanoparticles; for instance, transitions involved in absorption and emission spectra of NPs‐LA result in blueshifting with respect to the molecular solution of BT2, whereas redshifting is observed in NPs‐RP and NPs‐ME. Further, the results show that under infrared excitation, the aqueous suspensions of NPs‐LA exhibit the highest fluorescence induced by two‐photon absorption (≈790 GM at 740 nm), as well as the best photostability, compared with aqueous suspensions of NPs‐RP and NPs‐ME. The nanoparticles synthetized by the three aforementioned methods are employed as exogenous agents for the visualization of human cervical cancer cell line (HeLa) using confocal and two‐photon microscopy. Under similar experimental conditions, it is found that microscopy images of the best quality are obtained with NPs‐LA. These results show that laser ablation is a suitable technique for the fabrication of organic fluorescent nanoparticles used as contrast agents for in vitro fluorescence microscopy. |
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| Keywords: | microemulsion laser ablation organic nanoparticles reprecipitation two‐photon microscopy |
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