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Flavonoid profiles of immature and mature fruit tissues of Citrus grandis Osbeck (Dangyuja) and overall contribution to the antioxidant effect
Authors:Eun Ae Yu  Gon‐Sup Kim  Ji Eun Lee  Semin Park  Song Yi  Soo Jung Lee  Jae Hoon Kim  Jong Sung Jin  A M Abd El‐Aty  Jae‐Han Shim  Sung Chul Shin
Affiliation:1. Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju, Republic of Korea;2. Research Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea;3. Department of Food and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Republic of Korea;4. Faculty of Biotechnology, Citrus Genetic Resources Bank, College of Applied Life Science, Jeju National University, SARI, Jeju, Republic of Korea;5. Division of High Technology Materials Research, Busan Center, Korea Basic Science Institute (KBSI), Busan, Republic of Korea;6. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;7. Biotechnology Research Institute, College of Agriculture and Life Science, Chonnam National University, Yongbong‐ro 77, Buk‐gu, 500‐757 Gwangju, Republic of Korea
Abstract:Citrus fruits are a valuable functional food and their peel is used in East Asian folk medicine. In this study, the polar components of the fruit tissues of Citrus grandis Osbeck were analyzed using high‐performance liquid chromatography–tandem mass spectrometry and compared with reported data. Among the 13 characterized compounds, eight flavonoids and one coumarin were identified for the first time in fruit tissues. The total amount of the identified components was the largest for the immature fruit peel, followed by mature fruit peel, mature fruit flesh, and immature fruit flesh. Naringin (2) and neohesperidin (3) were particularly rich in all samples. The antioxidant activity of the flavonoids extracted from fruit tissues increased in a dose‐dependent manner. The activity of the fruit peels was significantly higher than that of the fruit flesh. Copyright © 2014 John Wiley & Sons, Ltd.
Keywords:Citrus grandis  Dangyuja  LC/MS/MS  flavonoids  antioxidant effect
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