Label‐Free Logic Modules and Two‐Layer Cascade Based on Stem‐Loop Probes Containing a G‐Quadruplex Domain |
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| Authors: | Yahui Guo Junjie Cheng Jine Wang Xiaodong Zhou Prof?Dr Jiming Hu Prof?Dr Renjun Pei |
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| Affiliation: | 1. Suzhou Key Laboratory of Nanotheranostics, Division of Nanobiomedicine, Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou Institute of Nano‐Tech and Nano‐Bionics, Chinese Academy of Sciences, Suzhou, 215123 (P.R. China);2. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry & Molecular Sciences, Wuhan University, Wuhan, 430072 (P.R. China) |
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| Abstract: | A simple, versatile, and label‐free DNA computing strategy was designed by using toehold‐mediated strand displacement and stem‐loop probes. A full set of logic gates (YES, NOT, OR, NAND, AND, INHIBIT, NOR, XOR, XNOR) and a two‐layer logic cascade were constructed. The probes contain a G‐quadruplex domain, which was blocked or unfolded through inputs initiating strand displacement and the obviously distinguishable light‐up fluorescent signal of G‐quadruplex/NMM complex was used as the output readout. The inputs are the disease‐specific nucleotide sequences with potential for clinic diagnosis. The developed versatile computing system based on our label‐free and modular strategy might be adapted in multi‐target diagnosis through DNA hybridization and aptamer‐target interaction. |
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| Keywords: | DNA computing G quadruplexes label‐free detection logic gates multiple‐input cascade |
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