Plasma lipid analysis by hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry |
| |
| Authors: | Kazuhiro Sonomura Shinobu Kudoh Taka‐Aki Sato Fumihiko Matsuda |
| |
| Affiliation: | 1. Center for Genomic Medicine, Kyoto University Graduate School of Medicine, Kyoto, Japan;2. Life Science Research Center, Technology Research Laboratory, Shimadzu Corporation, Kyoto, Japan;3. Pharmaceuticals and Life‐Science Division, Shimadzu Techno‐Research, Kyoto, Japan |
| |
| Abstract: | ![]()
A novel method for the analysis of endogenous lipids and related compounds was developed employing hydrophilic interaction liquid chromatography with electrospray ionization tandem mass spectrometry. A hydrophilic interaction liquid chromatography with carbamoyl stationary phase achieved clear separation of phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, ceramide, and mono‐hexsosyl ceramide groups with good peak area repeatability (RSD% < 10) and linearity (R2 > 0.99). The established method was applied to human plasma assays and a total of 117 endogenous lipids were successfully detected and reproducibly identified. In addition, we investigated the simultaneous detection of small polar metabolites such as amino and organic acids co‐existing in the same biological samples processed in a single analytical run with lipids. Our results show that hydrophilic interaction liquid chromatography is a useful tool for human plasma lipidome analysis and offers more comprehensive metabolome coverage. |
| |
| Keywords: | Electrospray ionization tandem mass spectrometry Hydrophilic interaction liquid chromatography Liquid chromatography Plasma Lipids |
|
|
