Chemical Synthesis of Asparagine‐Linked Archaeal N‐Glycan from Methanothermus fervidus |
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| Authors: | Someswara Rao Sanapala Prof. Dr. Suvarn S. Kulkarni |
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| Affiliation: | Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai, 400076 (India) |
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| Abstract: | Several N‐linked glycoproteins have been identified in archaea and there is growing evidence that the N‐glycan is involved in survival and functioning of archaea in extreme conditions. Chemical synthesis of the archaeal N‐glycans represents a crucial step towards understanding the putative function of protein glycosylation in archaea. Herein the first total synthesis of the archaeal L ‐asparagine linked hexasaccharide from Methanothermus fervidus is reported using a highly convergent [3+3] glycosylation approach in high overall yields. The synthesis relies on efficient preparation of regioselectively protected thioglycoside building blocks for orthogonal glycosylations and late stage N‐aspartylation. |
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| Keywords: | archaea glycosylation glycoproteins N‐aspartylation N‐glycans |
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