Multivalent Recognition of Concanavalin A by {Mo132} Glyconanocapsules—Toward Biomimetic Hybrid Multilayers |
| |
| Authors: | Dr Mihail Barboiu Dr Zineb Mouline Dr Mihaela Silion Erol Licsandru Prof Bogdan C Simionescu Dr Eugene Mahon Dr Mariana Pinteala |
| |
| Affiliation: | 1. Adaptive Supramolecular Nanosystems Group, Institut Européen des Membranes, ENSCM‐UMII‐CNRS UMR‐5635, Place Eugène Bataillon, CC 047, F‐34095, Montpellier (France), Fax: : (+33)?467149119;2. Centre of Advanced Research in Nanobioconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry 41A, Aleea Gr. Ghica Voda, Iasi (Romania) |
| |
| Abstract: | Herein, we consider Müller’s spherical, porous, anionic, molybdenum oxide based capsule, (NH4)42‐ {(MoVI)MoVI5O21(H2O)6}12{MoV2O4(CH3COO)}30]?10 CH3COONH4? 300 H2O≡(NH4)42? 1 a ?crystal ingredients≡ 1 , {Mo132}, as an effective sugar‐decorated nanoplatform for multivalent lectin recognition. The ion‐exchange of NH4+ ions of 1 with cationic‐sugars, D ‐mannose‐ammonium chloride ( 2 ) or D ‐glucose‐ammonium chloride ( 3 ) results in the formation of glyconanocapsules (NH4)42?n 2 n? 1 a and (NH4)42?m 3 m? 1 a . The Mannose (NH4)42?n 2 n? 1 a capsules bind selectively Concanavalin A (Con A) in aqueous solution, giving an association avidity constant of ${K{{{\rm multi}\hfill \atop {\rm a}\hfill}}}$ =4.6×104 M ?1 and an enhancement factor of β=K${{{{\rm multi}\hfill \atop {\rm a}\hfill}}}$ /K${{{{\rm mono}\hfill \atop {\rm ass}\hfill}}}$ =21.9, reminiscent of the formation of “glycoside clusters” on the external surface of glyconanocapsule. The glyconanocapsules (NH4)42?n 2 n? 1 a and (NH4)42?m 3 m? 1 a self‐assemble in “hybrid multilayers” by successive layer‐by‐layer deposition of (NH4)42?n 2 n? 1 a or (NH4)42?m 3 m? 1 a and Con A. These architectures, reminiscent of versatile mimics of artificial tissues, can be easily prepared and quantified by using quartz crystal microgravimetry (QCM). The “biomimetic hybrid multilayers” described here are stable under a continual water flow and they may serve as artificial networks for a greater depth of understanding of various biological mechanisms, which can directly benefit the fields of chemical separations, sensors or storage‐delivery devices. |
| |
| Keywords: | “ inorganic cells tissues” glyconano hybrids molybdates polyoxometalates sugar– protein interactions |
|
|
