| 微气泡激发的声微流对细胞声孔效应的影响 |
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| 引用本文： | 邱媛媛,张春兵,屠娟,章东.微气泡激发的声微流对细胞声孔效应的影响[J].声学学报,2012,37(1):91-96. |
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| 作者姓名： | 邱媛媛 张春兵 屠娟 章东 |
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| 作者单位： | 1 南京大学声学研究所 近代声学教育部重点实验室 南京 210093; |
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| 基金项目： | 国家科技部973计划(2011CB707900)、国家自然科学基金(10974093,11174141,11011130201,81171659)、江苏省自然科学基金(BE2010768)、江苏省高校自然科学研究面上项目(11KJB140005)、中央高校基本科研业务费专项资金(1103020402,1116020410,1112020401)和江苏高校优势学科建设工程资助项目。 |
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| 摘 要： | 理论及实验研究了微气泡激发的声微流对声孔效应的影响。实验采用低幅度(0.05~0.3MIPa)连续超声波信号照射MCF-7细胞,PEI:DNA的复合质粒和造影剂气泡的混合溶液,通过扫描电子显微镜测量细胞膜声孔效应。结果表明声孔大小随着激励声压的幅度和照射时间的增加而增大,平均孔径范围为100 nm~1.25μm。基于Marmottant微气泡振动模型的理论计算结果表明微气泡振动所产生的声微流引起的剪切力在低幅度超声引发声致穿孔作用中起着关键作用。
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| 收稿时间： | 2011-08-21 |
Effect of microbubble-induced microstreaming on the sonoporation |
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| Affiliation: | 1 Key Lab of Modern Acoustics, MOE, Institute of Acoustics, Nanjing University Nanjing 210093;2 School of Electronic information, Nangtong University Nangtong 226019;3 The Traditional Chinese Medicine Hospital of Jiangsu Province Nanjing 210029 |
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| Abstract: | In the present work.MCF-7 cells mixed with polyethylenimine:Deoxyribonucleic acid(DNA) complex and microbubbles were exposed to 1 MHz ultrasound with low acoustic driving pressures(0.05 ~ 0.3 MPa).The sonoporation pores generated on the cell membrane were examined with scanning electron microscopy.The results show that larger sonoporation pores would be generated with the increasing acoustic pressure or longer treatment time,which could be useful for the enhancement of DNA transfection efficiency,the mean diameters of pores ranged from 100 nm to 1.25μm. The calculated results based on Marmottant model indicate that the microstreaming-induced shear stress might be involved in the mechanisms of the low-intensity ultrasound induced sonoporation. |
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