| 延时提取正交发射MALDI技术研究 |
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| 作者单位： | 中国科学院安徽光学精密机械研究所环境光谱学实验室,合肥,230031;中国科学技术大学国家同步辐射实验室,合肥,230029 |
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| 摘 要： |
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| 关 键 词： | 反射式飞行时间质谱仪 延时提取 基质辅助激光解吸电离 正交发射 |
Research of Orthogonal-injection and Delayed-extraction MALDI |
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| Authors: | Gu Xuejun Fang Li Zheng Haiyang Zhou Liuzhu Wang Zhenya Zhang Weijun Sheng Liusi Zhang Yunwu |
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| Abstract: | An orthogonal injection (OI) home-made reflectror type time-of-flight (TOF) mass spectrometer has been constructed with a matrix-assisted laser desorption/ionization (MALDI) source. Ions generated by MALDI are measured using a pulsed voltage delayed extraction method. The laser used is a frequency quadrupled Nd:YAG laser with output at wavelength of 266 nm, the matrix used here is 2,5-dihydroxybenzonic acid (DHB), and the analytes are Malachite green and peptides. Measurements of resolving power and statistical evaluation of the mass accuracy are reported here. The results indicate that resolving power in the range of 3400 to 4000 (full width at half maximum), the average error of the mass accuracy is below 0.0075%, A perfectly linear (m/z)1/2 versus t plot is found. Finally, the initial velocity distribution of analyte and matrix ions in the range of 400~1000 m/s is measured. |
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| Keywords: | RTOF， Delayed extraction MALDI Orthogonal injection |
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