| 温度和离子对紫膜LB膜光循环的影响 |
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| 引用本文： | 胡坤生,王敖金,孙英.温度和离子对紫膜LB膜光循环的影响[J].影像科学与光化学,1993,11(2):30-35. |
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| 作者姓名： | 胡坤生 王敖金 孙英 |
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| 作者单位： | 中国科学院感光化学研究所, 北京 100101 |
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| 摘 要： | 用闪光动力学光谱仪测量了水平拉制的紫膜LB膜中菌紫质中间体M412的衰减过程,观察了温度和离子对M412衰减过程的影响。实验结果表明:在一定的温度范围内(10℃-60℃),随着温度的升高,M412的衰减速率加快。对M412s的衰减的抑制作用,La3+在低浓度时就很明显,而K+则在较高浓度时才表现出来,Ca2+的影响不明显;La3+对M412f的衰减无明显影响,K+和Ca2+则稍微加快了其速率,pH的变化(H+浓度)明显影响到M412的衰减速率,尤其在高pH情况,M412s的衰减比正常pH值时要慢一个数量级。
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| 关 键 词： | 紫膜 菌紫质 光化学循环 质子泵 LB膜 |
| 收稿时间： | 1992-03-16 |
THE EFFECT OF TEMPERATURE AND IONS ON PHOTOCYCLE OF PURPLE MEMBRANE LB FILM |
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| Hu KUN-SHENG,WANG AO-JIN,SUN YING.THE EFFECT OF TEMPERATURE AND IONS ON PHOTOCYCLE OF PURPLE MEMBRANE LB FILM[J].Imaging Science and Photochemistry,1993,11(2):30-35. |
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| Authors: | Hu KUN-SHENG WANG AO-JIN SUN YING |
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| Affiliation: | Institute of Biophysics, Academia Sinica, Beijing 100101, P.R. China |
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| Abstract: | The decay rate of bacteriorhodopsin photocycle intermediate M412 in purple membrane LB film treated by ions and temperature was studied. The results showed that the decay rate turned fast with increaseing temperature. The decay of M412s was inhibited by La3+ at low concentration, but by K+ at higher concentration. The decay of M412s was accelerated by K+ and Ca2+ except La3+. The change of pH affected the decay rate of M412, When pH value reached 11, the decay rate of M412s was much slower than that of normal pH value. |
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| Keywords: | purple membrane bacteriorhodopsin photocycle proton pump LB film |
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