Super‐Chelators for Advanced Protein Labeling in Living Cells |
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| Authors: | Karl Gatterdam Eike F Joest Dr Marina S Dietz Prof?Dr Mike Heilemann Prof?Dr Robert Tampé |
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| Affiliation: | 1. http://www.biochemie.uni‐frankfurt.de;2. Institute of Biochemistry, Biocenter, Goethe-University Frankfurt, Frankfurt/M, Germany;3. Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt/M, Germany |
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| Abstract: | Live‐cell labeling, super‐resolution microscopy, single‐molecule applications, protein localization, or chemically induced assembly are emerging approaches, which require specific and very small interaction pairs. The minimal disturbance of protein function is essential to derive unbiased insights into cellular processes. Herein, we define a new class of hexavalent N‐nitrilotriacetic acid (hexaNTA) chelators, displaying the highest affinity and stability of all NTA‐based small interaction pairs described so far. Coupled to bright organic fluorophores with fine‐tuned photophysical properties, the super‐chelator probes were delivered into human cells by chemically gated nanopores. These super‐chelators permit kinetic profiling, multiplexed labeling of His6‐ and His12‐tagged proteins as well as single‐molecule‐based super‐resolution imaging. |
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| Keywords: | cell delivery live-cell imaging metal-chelate complexes protein labeling super-resolution microscopy |
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