Electrochemical Behaviors of Resveratrol and Its Interaction with DNA |
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| Authors: | Lin Gao Xiaohan Wei Fei Wang Bingying Zhang |
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| Affiliation: | 1. Department of Material and Chemistry Engineering, Henan Institute of Engineering, Zhengzhou 450007, P. R. China;2. Henan Province Product Quality Supervision and Inspection Institute, Zhengzhou 450007, P. R. China |
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| Abstract: | Electrochemical behavior of resveratrol was studied in Britton‐Robinson (B‐R) buffer solution (pH = 4.0) at glassy carbon electrode (GCE) using cyclic voltammetry (CV). Resveratrol showed an irreversible anodic peak at 0.570 V which was involving one electron and one proton. Also, the interaction of resveratrol with double‐stranded fish sperm DNA was investigated by linear sweep voltammetry (LSV) and UV‐vis spectra. The results showed that peak potentials shifted to more positive value and peak currents decreased in electrochemical experiment and the maximum absorption decreased with red shift in UV‐vis spectra experiment with the addition of DNA, indicating the resveratrol interacted with DNA by intercalating into the double helix of DNA. Besides, the binding of resveratrol with DNA, analyzed in terms of the cooperative Hill model, yields the association constant Ka = 3.18 × 105 and a Hill coefficient m = 1.06. |
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| Keywords: | Anticancer herbal dresveratrolg Resveratrol DNA Interaction |
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