Nuclear Magnetic Resonance Structure Elucidation of Peptide b2 Ions |
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| Authors: | Pengyuan Liu Prof. Dr. R. Graham Cooks Prof. Dr. Hao Chen |
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| Affiliation: | 1. Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry & Edison Biotechnology Institute, Ohio University, Athens, OH 45701 (USA);2. Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907 (USA) http://aston.chem.purdue.edu/ |
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| Abstract: | ![]()
Tandem mass spectrometry (MS/MS) is powerful for chemical identification but it is still insufficient for explicit ion structure determination. A strategy is introduced to elucidate MS fragment ion structures using NMR spectroscopy for the first time. In our experiments, precursor ions are dissociated at atmospheric pressure and the resulting fragment ions are identified by mass spectrometry but collected outside the mass spectrometer, making the subsequent NMR measurements possible. This new strategy has been applied to determine the chemical structure of the characteristic b2 fragment ion, a subject of longstanding debate in MS‐based proteomics. |
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| Keywords: | fragments mass spectrometry NMR spectroscopy peptides structure elucidation |
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