EPR‐Based Approach for the Localization of Paramagnetic Metal Ions in Biomolecules |
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| Authors: | Dinar Abdullin Nicole Florin Dr Gregor Hagelueken Prof?Dr Olav Schiemann |
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| Affiliation: | Institute of Physical and Theoretical Chemistry, University of Bonn, Wegelerstrasse 12, 53115 Bonn (Germany) http://www.schiemann.uni‐bonn.de |
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| Abstract: | Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three‐dimensional fold of such biomolecules is therefore an important goal in understanding structure–function relationships. A trilateration approach for the localization of metal ions by means of long‐range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu2+ center of azurin, and factors affecting the precision of the method are discussed. |
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| Keywords: | binding sites copper EPR spectroscopy spin labels triangulation |
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