Chronological protein synthesis in regenerating rat liver |
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| Authors: | Hao Zhang Fuzheng Guo Lingyun Huang Xueyuan Xiao Dacheng He |
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| Affiliation: | Key Laboratory of Cell Proliferation and Regulation Biology Ministry of Education, Universities of the Confederated Institute for Proteomics, Beijing Normal University, Beijing, P. R. China |
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| Abstract: | Liver regeneration has been studied for decades; however, its regulation remains unclear. In this study, we report a dynamic tracing of protein synthesis in rat regenerating liver with a new proteomic technique, 35S in vivo labeling analysis for dynamic proteomics (SiLAD). Conventional proteomic techniques typically measure protein alteration in accumulated amounts. The SiLAD technique specifically detects protein synthesis velocity instead of accumulated amounts of protein through 35S pulse labeling of newly synthesized proteins, providing a direct way for analyzing protein synthesis variations. Consequently, protein synthesis within short as 30 min was visualized and protein regulations in the first 8 h of regenerating liver were dynamically traced. Further, the 3.5–5 h post partial hepatectomy (PHx) was shown to be an important regulatory turning point by acute regulation of many proteins in the initiation of liver regeneration. |
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| Keywords: | Dynamic proteomics Liver regeneration Protein synthesis velocity Pulse labeling |
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