HPLC‐fluorescence determination of thiol compounds in the serum of human male and female subjects using HILIC‐mode column |
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| Authors: | Yumiko Kono Hideaki Iizuka Muneki Isokawa Makoto Tsunoda Hideaki Ichiba Kiyomi Sadamoto Takeshi Fukushima |
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| Affiliation: | 1. Faculty of Pharmaceutical Sciences, Toho University, Chiba, Japan;2. Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan;3. Department of Clinical Pharmacy, Yokohama College of Pharmacy, Yokohama‐shi, Kanagawa, Japan |
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| Abstract: | High‐performance liquid chromatography–fluorescence detection using a hydrophilic interaction chromatography‐mode column (ZIC®‐HILIC) was used to determine four kinds of thiol compounds in human serum. Sera were obtained from 34 subjects for this study (17 male subjects aged 22–38 years and 17 female subjects aged 18–38 years). Serum cysteine, cysteinylglycine, glutathione, and γ‐glutamylcysteine, derivatized with ammonium 7‐fluoro‐2,1,3‐benzoxadiazole‐4‐sulfonate, were separated on the ZIC®‐HILIC column and quantified. The serum concentrations of cysteine, cysteinylglycine, glutathione and γ‐glutamylcysteine were 226 ± 4.7, 23.4 ± 1.3, 3.7 ± 0.2 and 3.2 ± 0.1 μm , respectively. In addition, the concentrations of serum thiol compounds from male subjects were significantly higher than those of the female subjects (p < 0.05). Copyright © 2014 John Wiley & Sons, Ltd. |
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| Keywords: | glutathione γ ‐glutamylcysteine ammonium 7‐fluoro‐2 1 3‐benzoxadiazole‐4‐sulfonate human serum gender difference |
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