Analysis and pharmacokinetic study of polyphyllin H in beagle dog plasma after oral administration of Rhizoma Paridis extracts by LC‐MS/MS |
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| Authors: | Mingrui Shen Yujing Zhai Sali Cao Jing Fu Xuechun Li Chunjing Yang Zhenwen Xia Yang Zhao Shangxin Li Ying Bai Dan Xue Jian Ni |
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| Affiliation: | 1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China;2. Beijing Experimental Vocational School, Beijing, China |
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| Abstract: | A highly sensitive, rapid assay method has been developed and validated for the analysis of polyphyllin H in beagle dog plasma with liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive‐ion mode. The assay procedure involves extraction of polyphyllin H and ginsenoside Re (IS) from beagle dog plasma. Chromatographic separation was carried out on an Agilent Zorbax XDB‐C18 (100 × 2.1 mm, 1.8μm) column by isocratic elution with acetonitrile and water (50:50, v/v) at a flow rate of 0.25 mL/min with a total run time of 2.5 min. The MS/MS ion transitions monitored were 870.46 → 869.6 for polyphyllin H and 947.12 → 969.60 for IS. Linear responses were obtained for polyphyllin H ranging from 1 to 50 ng/mL. The intra‐and inter‐day precisions (RSDs) were <1.77 and 3.39% and the extraction recovery ranged from 91.89 to 93.33% with RSD <2.68%. Stability studies showed that polyphyllin H was stable in the preparation and analytical process. The results indicated that the validated method was successfully used to determine the concentration–time profiles of polyphyllin H. Copyright © 2014 John Wiley & Sons, Ltd. |
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| Keywords: | polyphyllin H LC‐MS/MS pharmacokinetics beagle dog plasma |
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