Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy |
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| Authors: | Zhang Kai Li De-E Hong You-Li Zhu Pei-Ping Yuan Qing-Xi Huang Wan-Xia Gao Kun Zhou Hong-Zhang Wu Zi-Yu |
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| Affiliation: | [1]Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China [2]Key Laboratory of Zoological S ystematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [3]National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230026, China |
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| Abstract: | A penetrating view of the three-dimensional nanostructure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscopy are quite promising for insect morphology studies and, particularly, provide an attractive alternative to the destructive techniques used for investigating internal soft tissues. |
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| Keywords: | X-ray microscopy computed tomography synchrotron radiation source morphology |
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