Efficacious Anticancer Drug Delivery Mediated by a pH‐Sensitive Self‐Assembly of a Conserved Tripeptide Derived from Tyrosine Kinase NGF Receptor |
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| Authors: | Parikshit Moitra Krishan Kumar Prof. Paturu Kondaiah Prof. Santanu Bhattacharya |
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| Affiliation: | 1. Department of Organic Chemistry, Indian Institute of Science, Bangalore 560 012 (India);2. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560 012 (India) |
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| Abstract: | ![]()
We present herein a short tripeptide sequence (Lys–Phe–Gly or KFG) that is situated in the juxtamembrane region of the tyrosine kinase nerve growth factor (Trk NGF) receptors. KFG self‐assembles in water and shows a reversible and concentration‐dependent switching of nanostructures from nanospheres (vesicles) to nanotubes, as evidenced by dynamic light scattering, transmission electron microscopy, and atomic force microscopy. The morphology change was associated with a transition in the secondary structure. The tripeptide vesicles have inner aqueous compartments and are stable at pH 7.4 but rupture rapidly at pH≈6. The pH‐sensitive response of the vesicles was exploited for the delivery of a chemotherapeutic anticancer drug, doxorubicin, which resulted in enhanced cytotoxicity for both drug‐sensitive and drug‐resistant cells. Efficient intracellular release of the drug was confirmed by fluorescence‐activated cell sorting analysis, fluorescence microscopy, and confocal microscopy. |
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| Keywords: | drug delivery nanostructures peptides pH sensitivity self‐assembly |
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