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Polymersomes Prepared from Thermoresponsive Fluorescent Protein–Polymer Bioconjugates: Capture of and Report on Drug and Protein Payloads |
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| Authors: | Chin Ken Wong Alistair J Laos Dr Alexander H Soeriyadi Prof Jörg Wiedenmann Prof Paul M G Curmi Prof? J Justin Gooding Dr Christopher P Marquis Prof Martina H Stenzel Assoc?Prof Pall Thordarson |
| Affiliation: | 1. School of Chemistry, The Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio‐Nano Science and Technology, The University of New South Wales, Sydney, 2052, NSW (Australia);2. School of Chemistry, Centre for Advanced Macromolecular Design, The University of New South Wales, Sydney, 2052, NSW (Australia);3. Coral Reef Laboratory/Institute for Life Science (IfLS), University of Southampton, Southampton, SO14 3ZH/SO17 1BJ (UK);4. School of Physics, The University of New South Wales, Sydney, 2052, NSW (Australia);5. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney NSW 2052 (Australia) |
| Abstract: | Polymersomes provide a good platform for targeted drug delivery and the creation of complex (bio)catalytically active systems for research in synthetic biology. To realize these applications requires both spatial control over the encapsulation components in these polymersomes and a means to report where the components are in the polymersomes. To address these twin challenges, we synthesized the protein–polymer bioconjugate PNIPAM‐b‐amilFP497 composed of thermoresponsive poly(N‐isopropylacrylamide) (PNIPAM) and a green‐fluorescent protein variant (amilFP497). Above 37 °C, this bioconjugate forms polymersomes that can (co‐)encapsulate the fluorescent drug doxorubicin and the fluorescent light‐harvesting protein phycoerythrin 545 (PE545). Using fluorescence lifetime imaging microscopy and Förster resonance energy transfer (FLIM‐FRET), we can distinguish the co‐encapsulated PE545 protein inside the polymersome membrane while doxorubicin is found both in the polymersome core and membrane. |
| Keywords: | compartmentalization encapsulation FRET polymersomes protein modifications |
