Impedimetric DNA‐Based Biosensor for Silver Ions Detection with Hemin/G‐Quadruplex Nanowire as Enhancer |
| |
| Authors: | Guangpeng Liu Yali Yuan Shiqiang Wei Dujian Zhang |
| |
| Affiliation: | 1. College of resources and environments, Southwest University, Chongqing 400715, P.?R. China;2. Key Laboratory of Luminescent and Real‐Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P.?R. China |
| |
| Abstract: | A DNA‐based biosensor was reported for detection of silver ions (Ag+) by electrochemical impedance spectroscopy (EIS) with Fe(CN)6]4?/3? as redox probe and hybridization chain reaction (HCR) induced hemin/G‐quadruplex nanowire as enhanced label. In the present of target Ag+, Ag+ interacted with cytosine‐cytosine (C? C) mismatch to form the stable C? Ag+? C complex with the aim of immobilizing the primer DNA on electrode, which thus triggered the HCR to form inert hemin/G‐quadruplex nanowire with an amplified EIS signal. As a result, the DNA biosensor showed a high sensitivity with the concentration range spanning from 0.1 nM to 100 µM and a detection limit of 0.05 nM. |
| |
| Keywords: | Electrochemical impedance spectroscopy Hemin/G‐quadruplex nanowire DNA biosensor Silver ion Hybridization chain reaction |
|
|
