Commercial Screen‐Printed Gold Electrodes for the Detection and Quantification of Aminothiols in Human Plasma by Liquid Chromatography with Electrochemical Detection |
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| Authors: | Santiago Cavanillas Núria Serrano José Manuel Díaz‐Cruz Cristina Ariño Miquel Esteban |
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| Affiliation: | Departament de Química Analítica, Universitat de Barcelona, Martí i Franquès, 1‐11, E‐08028 – Barcelona (Spain) phone: (+34) 93?403?91 16, fax: (+34) 93?402?12?33 |
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| Abstract: | In order to show the possibilities of screen‐printed electrodes in HPLC detection, a method is presented for routine quantification of aminothiols in human plasma. It uses commercial gold screen‐printed electrodes and provides a rapid, simple and economical procedure with a disposable detector and without any additional electrode. By working at 98 : 2 (0.05 % trifluoroacetic acid: methanol) and 0.9 V, it allows us to determine cysteine (Cys), cysteinyl‐glycine (Cys‐Gly), and homocysteine (HCys) at μmol/L levels. The method was validated, and linearity range, detection and quantification limits, precision and accuracy were evaluated by external calibration and by using N‐acetylcysteine as internal standard, which exhibited better precision. |
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| Keywords: | Liquid chromatography Electrochemical detection Gold screen‐printed electrode Aminothiols Human plasma |
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