Dynamic Expression of DNA Complexation with Self‐assembled Biomolecular Clusters |
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| Authors: | Eline Bartolami Dr. Yannick Bessin Dr. Virginie Gervais Prof. Dr. Pascal Dumy Dr. Sébastien Ulrich |
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| Affiliation: | 1. Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université Montpellier, ENSCM, Ecole Nationale Supérieure de Chimie de Montpellier, 8 Rue de l'Ecole Normale, 34296 Montpellier cedex 5 (France);2. CNRS;3. IPBS (Institut de Pharmacologie et de Biologie Structurale), 205 route de Narbonne, BP64182, 31077 Toulouse (France);4. Université de Toulouse;5. UPS;6. IPBS, 31077 Toulouse (France) |
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| Abstract: | We report herein the implementation of a dynamic covalent chemistry approach to the generation of multivalent clusters for DNA recognition. We show that biomolecular clusters can be expressed in situ by a programmed self‐assembly process using chemoselective ligations. The cationic clusters are shown, by fluorescence displacement assay, gel electrophoresis and isothermal titration calorimetry, to effectively complex DNA through multivalent interactions. The reversibility of the ligation was exploited to demonstrate that template effects occur, whereby DNA imposes component selection in order to favor the most active DNA‐binding clusters. Furthermore, we show that a chemical effector can be used to trigger DNA release through component exchange reactions. |
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| Keywords: | bioconjugates DNA recognition dynamic covalent chemistry multivalency self‐assembly |
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