An LC‐MS/MS method for determination of calceorioside B with cardiomyocyte protective activity in rat plasma and application to a pharmacokinetic study |
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| Authors: | Fan Yang Yanqing Song Sixi Zhang Wei Zhou |
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| Affiliation: | 1. Department of Pediatric Surgery, the First Hospital of Jilin University, Changchun, China;2. Department of Pharmacy, the First Hospital of Jilin University, Changchun, China |
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| Abstract: | A simple, sensitive and rapid liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method was developed and validated for the determination of calceorioside B (CLB) in rat plasma. Detection was performed on a Thermo Scientific Hypersil Gold chromatography column using isocratic elution with a mobile phase of methanol–5 m m ammonium acetate–formic acid (70:30:0.1, v/v/v). Mass spectrometry was performed in selection reaction monitoring mode using a positive electrospray ionization interface. Good linearity was found for CLB in plasma in the linear range of 1.00–500 ng/mL (r > 0.9960). The validated method was successfully applied to the pharmacokinetic study of CLB in rats. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | calceorioside B LC‐MS/MS rat plasma pharmacokinetics |
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