Development of Electrochemical Paper‐based Glucose Sensor Using Cellulose‐4‐aminophenylboronic Acid‐modified Screen‐printed Carbon Electrode |
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| Authors: | Tipawan Rungsawang Eakkasit Punrat Jaclyn Adkins Charles Henry Orawon Chailapakul |
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| Affiliation: | 1. Program in Biotechnology, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330 Thailand;2. Electrochemistry and Optical Spectroscopy Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330 Thailand, Tel.: +66‐2‐218‐7615;3. Fax: +66‐2‐254‐1309;4. Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523‐1872 USA, Tel.: +1‐970‐491‐2852;5. Fax: +1‐970‐491‐1801;6. Center of Excellence for Petroleum, Petrochemicals, and Advanced Materials, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand |
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| Abstract: | The present work describes the fabrication of paper‐based analytical devices (μPADs) by immobilization of glucose oxidase onto the screen printed carbon electrodes (SPCEs) for the electrochemical glucose detection. The sensitivity towards glucose was improved by using a SPCE prepared from homemade carbon ink mixed with cellulose acetate. In addition, 4‐aminophenylboronic acid (4‐APBA) was used as a redox mediator giving a lower detection potential for improvement selectivity. Under optimized condition, the detection limit was 0.86 mM. The proposed device was applied in real samples. This μPAD has many advantages including low sample consumption, rapid analysis method, and low device cost. |
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| Keywords: | Paper‐based analytical device 4‐aminophenylboronic acid (4‐APBA) Glucose Biosensor |
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