Scanning Electrochemical Microscopy for Electrochemical Detection of Single‐base Mismatches by Tagging Ferrocenecarboxylic Acid as a Redox Probe to DNA |
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| Authors: | Masoud A Mehrgardi Mir F Mousavi |
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| Affiliation: | 1. Department of Chemistry, University of Isfahan, Isfahan 81746‐73441, Iran;2. Department of Chemistry, Faculty of Science, Tarbiat Modares University, Tehran 14115‐175, Iran |
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| Abstract: | Scanning electrochemical microscopy (SECM) was employed for sensitive detection of single base mismatches (SBMs) in a sandwiched dsDNA. Ferrocenecarboxylic acid (Fc), covalently conjugated to the dsDNA, was oxidized to Fc+ via the DNA‐mediated charge transfer from the underlying gold substrate, and reduced back to Fc by SECM tip generated ferrocyanide. The electrocatalytic oxidation of SECM tip‐generated ferrocyanide was sensitive to presence, as well as the type of SBMs. Apparent standard rate constants (k0app) values for different SBMs, both near the electrode surface and far from it, were evaluated by SECM. The method can detect SBMs independent of their position in dsDNA. |
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| Keywords: | Single‐base mismatch (SBM) Ferrocenecarboxylic acid Scanning electrochemical microscopy DNA‐mediated charge transfer Bimolecular electron transfer |
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