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| 高效凝胶渗透色谱测定明胶的分子量分布 | |
| 引用本文： | 陈祥舫,彭必先. 高效凝胶渗透色谱测定明胶的分子量分布[J]. 影像科学与光化学, 1984, 2(1): 7-14. DOI: 10.7517/j.issn.1674-0475.1984.01.7 |
| 作者姓名： | 陈祥舫 彭必先 |
| 作者单位： | 中国科学院感光化学研究所 |
| 摘 要： | 本工作用高效凝胶渗透色谱测定了明胶的分子量及其分布。使用了三根串联的μ-Bondagel E-linear柱,用2%的十二烷基硫酸钠作为淋洗剂,采用α、β和γ三种胶样作为标准样品。测定了惰胶和改性胶的MN、MW、MZ和Mη。估计并讨论了改性胶在制造过程中的降解量。 |
| 收稿时间： | 1983-04-13 |
DETERMINATION OF MOLECULAR WEIGHT DISTRIBUTION OF GELATINS BY HIGH PERFORMANCE GEL PERMEATION CHROMATOGRAPHY |
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| CHEN XIANG-FANG,PENG BI-XIAN. DETERMINATION OF MOLECULAR WEIGHT DISTRIBUTION OF GELATINS BY HIGH PERFORMANCE GEL PERMEATION CHROMATOGRAPHY[J]. Imaging Science and Photochemistry, 1984, 2(1): 7-14. DOI: 10.7517/j.issn.1674-0475.1984.01.7 | |
| Authors: | CHEN XIANG-FANG PENG BI-XIAN |
| Affiliation: | Institute of Photographic Chemistry, Academia Sinica |
| Abstract: | In present work the molecular weight distribution (MWD) of inert gelatins and modified gelatins was studied by High Performance Gel Permeation Chromatography. Three columns of μ-Bondagel E-linear was connected in series and used for separation. Sodium dodecyl sulfate (2%) was used as eluent. The α, β and γ components were used as standard samples. The MN, MW, MZ and Mη of inert and modified gelatins were measured and compared with the calculated values. The degree of degradation of the parent gelatin during it’s modification process under high pH was determined and the results were discussed. |
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