Ion‐pair cloud‐point extraction: A new method for the determination of water‐soluble vitamins in plasma and urine |
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| Authors: | Rouhollah Heydari Najmeh S Elyasi |
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| Affiliation: | 1. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, , Khorramabad, Iran;2. Department of Chemistry, Khouzestan Science & Research Branch, Islamic Azad University, , Ahvaz, Iran |
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| Abstract: | A novel, simple, and effective ion‐pair cloud‐point extraction coupled with a gradient high‐performance liquid chromatography method was developed for determination of thiamine (vitamin B1), niacinamide (vitamin B3), pyridoxine (vitamin B6), and riboflavin (vitamin B2) in plasma and urine samples. The extraction and separation of vitamins were achieved based on an ion‐pair formation approach between these ionizable analytes and 1‐heptanesulfonic acid sodium salt as an ion‐pairing agent. Influential variables on the ion‐pair cloud‐point extraction efficiency, such as the ion‐pairing agent concentration, ionic strength, pH, volume of Triton X‐100, extraction temperature, and incubation time have been fully evaluated and optimized. Water‐soluble vitamins were successfully extracted by 1‐heptanesulfonic acid sodium salt (0.2% w/v) as ion‐pairing agent with Triton X‐100 (4% w/v) as surfactant phase at 50°C for 10 min. The calibration curves showed good linearity (r2 > 0.9916) and precision in the concentration ranges of 1‐50 μg/mL for thiamine and niacinamide, 5–100 μg/mL for pyridoxine, and 0.5–20 μg/mL for riboflavin. The recoveries were in the range of 78.0–88.0% with relative standard deviations ranging from 6.2 to 8.2%. |
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| Keywords: | Cloud‐point extraction Ion‐pairing agents Plasma Urine Water‐soluble vitamins |
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