Selective retention of basic compounds by metal aquo‐ion affinity chromatography |
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| Authors: | Yoshiki Asakawa Eiichi Yamamoto Naoki Asakawa |
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| Affiliation: | 1. Analysis Group, Tsukuba Division, Sunplanet Co., Ltd., Tokodai, Tsukuba, Ibaraki, Japan;2. Global Formulation Japan, Pharmaceutical Scientific and Technology Core Function Unit, Eisai Product Creation Systems, Eisai Co., Ltd., Tokodai, Tsukuba, Ibaraki, Japan;3. Pharmaceutical Scientific and Technology Core Function Unit, Eisai Product Creation Systems, Eisai Co., Ltd., Tokodai, Tsukuba, Ibaraki, Japan |
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| Abstract: | A novel metal aquo‐ion affinity chromatography has been developed for the analysis of basic compounds using heat‐treated silica gel containing hydrated metal cations (metal aquo‐ions) as the packing material. The packing materials of the metal aquo‐ion affinity chromatography were prepared by the immobilization of a single metal component such as Fe(III), Al(III), Ag(I), and Ni(II) on silica gel followed by extensive heat treatment. The immobilized metals form aquo‐ions to present cation‐exchange ability for basic analytes and the cation‐exchange ability for basic analytes depends on pKa of the immobilized metal species. In the present study, to evaluate the retention characteristics of metal aquo‐ion affinity chromatography, the on‐line solid‐phase extraction of drugs was investigated. Obtained data clearly evidence the selective retention capability of metal aquo‐ion affinity chromatography for basic analytes with sufficient capacity. |
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| Keywords: | Basic compounds Heat‐treated silica gel High‐performance liquid chromatography Metal aquo‐ions |
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