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EFFECT OF SPHERICAL ABERRATION INTRODUCED BY WATER SOLUTION ON TRAPPING FORCE |
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| Authors: | Yao Xin-cheng Li Zhao-lin Guo Hong-lian Cheng Bing-ying Han Xue-hai and Zhang Dao-zhong |
| Affiliation: | Optical Physics Laboratory, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080, China; State Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China |
| Abstract: | Trapping force of an optical tweezers system with an oil immersion objective is calculated with a ray-optics model. Results indicate that the trapping force will be decreased as a result of the introduction of spherical aberration, which is caused by the refractive mismatch between objective oil and water, when the sample manipulated is suspended in a water solution. The effect of spherical aberration will be serious when the detection depth of the optical tweezers is enhanced. |
| Keywords: | spherical aberration optical tweezers trapping force |
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