1. Institute of Industrial Science, The University of Tokyo, Meguro‐Ku, Tokyo, Japan;2. Institute of Industrial Science, The University of Tokyo, Tokyo, Japan;3. Hanyang University, Gyeonggi, South Korea;4. Takeuchi Biohybrid Innovation Project, xploratory Research for Advanced Technology (ERATO), Tokyo, Japan
Abstract:
A collagen sheet with highly aligned collagen fibers is fabricated by continuous cyclic stretch. The rearrangement of the collagen fibers depends on the different process parameters of the cyclic stretch, including magnitude, frequency, and period of stretch. The collagen fibers are aligned perpendicularly to the direction of the stretch. Corneal stromal cells and smooth muscle cells cultivated on the highly aligned collagen sheet show alignment along the collagen fibers without the stretch during culture. Thus, the sheet can be a suitable scaffold for use in regenerative medicine.
