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利用副干酪乳杆菌发酵风味鱿鱼制品条件研究
引用本文:黄乐丹 吴祖芳 张林楠 白政泽 翁佩芳.利用副干酪乳杆菌发酵风味鱿鱼制品条件研究[J].宁波大学学报(理工版),2017,0(3):6-11.
作者姓名:黄乐丹  吴祖芳  张林楠  白政泽  翁佩芳
作者单位:1.宁波大学 海洋学院, 浙江 宁波 315211; 2.宁波飞润海洋生物科技有限公司, 浙江 宁波 315700
摘    要:为进一步提高鱿鱼的食用品质, 以北太鱿鱼作为副干酪乳杆菌Lpsi的发酵基质, 研究料液比、菌株接种量、蔗糖添加量、发酵时间、发酵温度对鱿鱼发酵效果的影响. 采用响应面法对发酵条件进行优化, 得到副干酪乳杆菌发酵鱿鱼的最佳优化条件为: 料液比1:1.92、发酵温度31.42℃、加糖量3.16%、接种量4%, 发酵时间48h. 在此条件下, 鱿鱼发酵液的实际氨基氮含量达到0.63g?L-1. 鱿鱼经过副干酪乳杆菌蛋白酶的分解作用, 呈味氨基酸含量上升, 提高了人体必需氨基酸含量及易消化吸收性, 使其营养性和保健性得到进一步增强.

关 键 词:鱿鱼  副干酪乳杆菌Lpsi  发酵  响应面法

Study on fermentation conditions of flavor squid products by Lactobacillus paracasei
HUANG Le-dan,WU Zu-fang,ZHANG Lin-nan,BAI Zheng-ze,WENG Pei-fang.Study on fermentation conditions of flavor squid products by Lactobacillus paracasei[J].Journal of Ningbo University(Natural Science and Engineering Edition),2017,0(3):6-11.
Authors:HUANG Le-dan  WU Zu-fang  ZHANG Lin-nan  BAI Zheng-ze  WENG Pei-fang
Affiliation:1.School of Marine Science, Ningbo University, Ningbo 315211, China; 2.Feirun Marine Biotechnology Co., Ltd., Ningbo 315700, China
Abstract:In order to further improve the edible quality of North Pacific squid products, North Pacific squid was used as the fermentation substrate of Lactobacillus paracasei. The effect of solid to liquid ratio, sucrose concentration, the quantity of inoculation of lactobacillus, fermentation time and fermentation temperature in fermented squid was evaluated. The response surface methodology was used to optimize the fermentation conditions and the optimal conditions for solid to liquid ratio, fermentation temperature, sucrose concentration, inoculation amount and fermentation time were determined as 1:1.92, 31.42℃, 3.16%, 4% and 48h, respectively. Experiments carried out under these conditions led to an amino nitrogen content of 0.63g?L-1. Decomposed by Lactobacillus paracasei protease, the content of amino acids in North Pacific squid increased, and its digestion and absorption and the content of essential amino acids in the human body also increased. In addition, its nutritional and health-care properties were further enhanced.
Keywords:squid  Lactobacillus paracasei Lactobacillus paracasei  fermentation  response surface methodology
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