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Polyamide as an efficient sorbent for simultaneous interference-free determination of three Sudan dyes in saffron and urine using high-performance liquid chromatography-ultra violet detection北大核心CSCD
引用本文:Iman SAEIDI,Behruz BARFI,Moazameh PAYROVI,Javad FEIZY,Hojat A SHEIBANI,Mina MIRI,Farahnaz GHOLLASI MOUD. Polyamide as an efficient sorbent for simultaneous interference-free determination of three Sudan dyes in saffron and urine using high-performance liquid chromatography-ultra violet detection北大核心CSCD[J]. 色谱, 2015, 33(1): 29-34. DOI: 10.3724/SP.J.1123.2014.08029
作者姓名:Iman SAEIDI  Behruz BARFI  Moazameh PAYROVI  Javad FEIZY  Hojat A SHEIBANI  Mina MIRI  Farahnaz GHOLLASI MOUD
作者单位:1. Iranian National Standards Organization, Mashhad 91735-344, Iran;2. Department of Chemistry, University of Semnan, Semnan 35131-19111, Iran;3. Department of Chemistry, University of Mazandran, Babolsar 47416-95447, Iran;4. Testa Quality Control Laboratory, Mashhad 91895/157-134, Iran;5. Birjand University of Technology, Birjand 97198-66981, Iran
基金项目:The authors are grateful to the financial support of Institute of Standards and Industrial Research of Iran (Khorasan-e-RazaviProvince).
摘    要:With polyamide( PA)as an efficient sorbent for solid phase extraction( SPE)of Sudan dyes II,III and Red 7B from saffron and urine,their determination by HPLC was performed. The optimum conditions for SPE were achieved using 7 mL methanol/water( 1:9,v/v,pH 7)as the washing solvent and 3 mL tetrahydrofu-ran for elution. Good clean-up and high( above 90%)recoveries were observed for all the analytes. The opti-mized mobile phase composition for HPLC analysis of these compounds was methanol-water( 70:30,v/v). The SPE parameters,such as the maximum loading capacity and breakthrough volume,were also determined for each analyte. The limits of detection( LODs),limits of quantification( LOQs),linear ranges and recoveries for the analytes were 4. 6-6. 6 μg/L,13. 0-19. 8 μg/L,13. 0-5 000 μg/L( r2> 0. 99)and 92. 5% -113. 4%,respec-tively. The precisions( RSDs)of the overall analytical procedure,estimated by five replicate measurements for Sudan II,III and Red 7B in saffron and urine samples were 2. 3%,1. 8% and 3. 6%,respectively. The developed method is simple and successful in the application to the determination of Sudan dyes in saffron and urine sam-ples with HPLC coupled with UV detection.

关 键 词:high-performanceliquidchromatography(HPLC)  polyamide(PA)  saffron  solidphaseextraction(SPE)  Sudandyes  urine  
收稿时间:2014-08-27

Polyamide as an efficient sorbent for simultaneous interference-free determination of three Sudan dyes in saffron and urine using high-performance liquid chromatography-ultra violet detection
Iman SAEIDI,Behruz BARFI,Moazameh PAYROVI,Javad FEIZY,Hojat A SHEIBANI,Mina MIRI,Farahnaz GHOLLASI MOUD. Polyamide as an efficient sorbent for simultaneous interference-free determination of three Sudan dyes in saffron and urine using high-performance liquid chromatography-ultra violet detection[J]. Chinese journal of chromatography, 2015, 33(1): 29-34. DOI: 10.3724/SP.J.1123.2014.08029
Authors:Iman SAEIDI  Behruz BARFI  Moazameh PAYROVI  Javad FEIZY  Hojat A SHEIBANI  Mina MIRI  Farahnaz GHOLLASI MOUD
Affiliation:1. Iranian National Standards Organization, Mashhad 91735-344, Iran;2. Department of Chemistry, University of Semnan, Semnan 35131-19111, Iran;3. Department of Chemistry, University of Mazandran, Babolsar 47416-95447, Iran;4. Testa Quality Control Laboratory, Mashhad 91895/157-134, Iran;5. Birjand University of Technology, Birjand 97198-66981, Iran
Abstract:With polyamide (PA) as an efficient sorbent for solid phase extraction (SPE) of Sudan dyes II, III and Red 7B from saffron and urine, their determination by HPLC was performed. The optimum conditions for SPE were achieved using 7 mL methanol/water (1: 9, v/v, pH 7) as the washing solvent and 3 mL tetrahydrofuran for elution. Good clean-up and high (above 90%) recoveries were observed for all the analytes. The optimized mobile phase composition for HPLC analysis of these compounds was methanol-water (70: 30, v/v). The SPE parameters, such as the maximum loading capacity and breakthrough volume, were also determined for each analyte. The limits of detection (LODs), limits of quantification (LOQs), linear ranges and recoveries for the analytes were 4.6-6.6 μg/L, 13.0-19.8 μg/L, 13.0-5000 μg/L (r2 > 0.99) and 92.5%-113.4%, respectively. The precisions (RSDs) of the overall analytical procedure, estimated by five replicate measurements for Sudan II, III and Red 7B in saffron and urine samples were 2.3%, 1.8% and 3.6%, respectively. The developed method is simple and successful in the application to the determination of Sudan dyes in saffron and urine samples with HPLC coupled with UV detection.
Keywords:solid phase extraction(SPE)  high-performance liquid chromatography(HPLC)  Sudan dyes  saffron  urine  polyamide(PA)
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