| L-Cys/MPA共修饰CdTe量子点:制备、表征及其在细胞标记中的应用 |
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| 引用本文： | 丁兆强,权静,聂华丽,朱利民.L-Cys/MPA共修饰CdTe量子点:制备、表征及其在细胞标记中的应用[J].影像科学与光化学,2015,33(2):126-135. |
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| 作者姓名： | 丁兆强 权静 聂华丽 朱利民 |
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| 作者单位： | 东华大学 化学化工与生物工程学院, 上海 201620 |
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| 基金项目： | 国家自然科学基金项目，中央高校基本科研业务经费专项资金资助 |
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| 摘 要： | 以L-半胱氨酸(L-Cys)和巯基丙酸(MPA)为共修饰剂在水相中快速合成高质量CdTe量子点.通过紫外-可见分光光谱、荧光光谱、荧光寿命衰减曲线、透射电子显微图片、XRD图谱等相关方法对产物进行表征.调节L-Cys和MPA的摩尔比,对CdTe量子点的生长速率和光学特性有明显影响.结果证实:与单一修饰剂L-Cys或MPA相比,L-Cys/MPA共修饰CdTe量子点具有较快的荧光发射峰红移速率,且其粒径分布均一、稳定性强.50L-CdTe(L-Cys与MPA摩尔比为50%)的荧光量子产率达到66.4%,75L-CdTe(L-Cys与MPA摩尔比为75%)的荧光寿命为46.8 ns.细胞毒性实验证明75L-CdTe量子点对SiHa细胞毒性较小,细胞存活率为75%~95%.进一步将其用于标记细胞,表明75L-CdTe量子点能有效地对SiHa细胞进行荧光标记.L-Cys/MPA共修饰CdTe量子点具有良好的荧光特性和生物相容性,在生物医药领域具有重要的应用价值.
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| 关 键 词： | 细胞标记 量子点 共修饰 L-半胱氨酸 巯基丙酸 |
| 收稿时间： | 2014-07-31 |
CdTe Quantum Dots Using L-Cys/MPA as Mixed-ligands: Preparation,Characterization and Application in Cell Labeling |
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| DING Zhaoqiang,QUAN Jing,NIE Huali,ZHU Limin.CdTe Quantum Dots Using L-Cys/MPA as Mixed-ligands: Preparation,Characterization and Application in Cell Labeling[J].Imaging Science and Photochemistry,2015,33(2):126-135. |
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| Authors: | DING Zhaoqiang QUAN Jing NIE Huali ZHU Limin |
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| Affiliation: | College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China |
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| Abstract: | High-quality CdTe quantum dots (QDs) were rapidly synthesized in aqueous phase using both L-Cysteine (L-Cys) and mercaptopropionic acid (MPA) as mixed-ligands. Different technologies including UV-Vis spectra, fluorescence spectra, luminescence decay curves, transmission electron microscopy and X-ray diffraction analysis were employed to characterize the prepared QDs. The ratio of L-Cys and MPA was found to have influence on the growth kinetics and optical properties of the obtained QDs. Compared to single ligand of L-Cys or MPA, L-Cys/MPA as mixed-ligands could accelerate the red shifted rate of fluorescence emission peak wavelength effectively. CdTe QDs capped with mixed-ligands exhibited a high uniformity in diameter and an excellent photostability. 50L-CdTe (L-Cys/MPA molar ratio was 50%) held a high quantum yield of 66.4% and 75L-CdTe (L-Cys/MPA molar ratio was 75%) exhibited a luminescence decay lifetime of 46.8 ns. MTT assays showed that 75L-CdTe with emission of 615 nm maintained 75%-95% SiHa cell viability. Further study of labeling SiHa cell with the as-prepared 75L-CdTe indicated that the QDs are excellent biomarkers for SiHa cell. L-Cys and MPA as mixed-ligands with proper design can be employed to fabricate CdTe QDs with superior fluorescence properties and biocompatibility, which have potential in biological and medical fields as biomarker-candidates. |
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| Keywords: | cell labeling quantum dots mixed ligands L-Cysteine mercaptopropionic acid |
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