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化学进展 2010, Vol. 22 Issue (01): 163-172 前一篇   后一篇

• 综述与评论 •

微生物代谢物组学的研究方法与进展*

王智文;马向辉;陈洵;赵学明;陈涛**   

  1. （天津大学化工学院生物工程系代谢工程和系统生物学实验室 系统生物工程教育部重点实验室 爱丁堡大学-天津大学系统生物学与合成生物学联合研究中心 天津 300072）
  • 收稿日期:2009-01-19 修回日期:2009-04-07 出版日期:2010-01-24 发布日期:2010-01-07
  • 通讯作者: 陈涛 E-mail:chentao@tju.edu.cn
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Current Methods and Advances in Microbial Metabolomics

Wang Zhiwen;  Ma Xianghui;  Chen Xun;  Zhao Xueming;  Chen Tao**   

  1. (Metabolic Engineering and Systems Biology Laboratory, Key Laboratory of Systems Bioengineering Ministry of Education, Edinburgh-Tianjin Joint Research Centre for Systems Biology and Synthetic Biology, Department of Biological Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China)
  • Received:2009-01-19 Revised:2009-04-07 Online:2010-01-24 Published:2010-01-07
  • Contact: Chen Tao E-mail:chentao@tju.edu.cn

代谢物组学、基因组学、转录组学和蛋白质组学是系统生物学研究的重要组成部分。近年来，在代谢物组学领域，微生物代谢物组学的研究受到人们的重视，成为研究的热点。本文综述了微生物代谢物组学的研究方法，包括样品处理、分析平台、数据处理和生物学解释等，并讨论了微生物代谢物组学在代谢工程方面的应用潜力，以及微生物代谢物组学的研究前景和所面临的挑战。

关键词： 微生物代谢组学, 系统生物学, 数据挖掘, 代谢物, 代谢工程

In the post-genomic era, metabolomics(or metabonomics), genomics, transcriptomics and proteomics have become important tools in systems biology. In metabolome analysis field, microbial metabolomics has received much attention in recent years mainly because it supports and complements a wide range of microbial research areas from new drug discovery efforts to metabolic engineering. This review is mainly focused on the current methods and advances in microbial metabolomics, trying to direct the reader to the main approaches for microbial metabolome analysis, reviewing the developments of methodologies in microbial metabolomics. These developments range from microorganism cultivation, sample preparation, analytical platform and data analysis to biological interpretation. Apart from the description of the developments, we will try to discuss the potential of microbial metabolomics in metabolic engineering field, research prospect and challenge of microbial metabolomics in the future.

Contents
1 Introduction
2 Research methods of microbial metabolomics
2.1 Cell cultivation
2.2 Sample treatment
2.3 Analytical platform of microbial metabolomics
2.4 Data mining
3 Microbial metabolomics and metabolic engineering
4 Prospects

Key words: microbial metabolomics, systems biology, data mining, metabolite, metabolic engineering

中图分类号: 

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