| 碳点-钙黄绿素荧光共振能量转移体系在山奈素检测中的应用研究 |
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| 基金项目:国家自然科学基金（61264007）；广西自然基金（2013GXNSFAA019034） |
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| 中文摘要:研究了碳点（CDs）-钙黄绿素（CA）能量转移体系，并利用该体系建立了测定山奈素（KF）的新方法。在λex=320 nm下，pH 8.0的磷酸氢二钠-柠檬酸缓冲液中，CDs与CA能发生有效的能量转移，使得CA的荧光增强，而KF的加入则可有效猝灭CA的荧光，且在一定范围内CA的荧光猝灭值与KF的加入量呈良好的线性关系，基于此建立了测定KF的新方法。在最佳实验条件下，方法的线性范围为1.0×10-8～ 3.4×10-6 mol/L,检出限为3.33×10-9 mol/L。方法应用于复方金银花颗粒中KF含量的测定，回收率为98.4% ～ 103.5% ，RSD（n=6）不大于2.7% 。 |
| 中文关键词:碳点 钙黄绿素 荧光共振能量转移 山奈素 |
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| Study on the Fluorescence Resonance Energy Transfer System of Carbon Dots-Calcein and Its Application in the Detection of Kaempferol |
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| Abstract:A fluorescence resonance energy transfer （FRET） system between fluorescence carbon dots （CDs,donor） and calcein （CA,acceptor） was constructed,and a novel platform for the sensitive and selective determination of kaempferol （KF） was proposed.It was found that in pH 8.0 sodium hydrogen phosphate-citric acid buffer medium,effective energy transfer from CDs to CA occurred,which resulted in a great enhancement of the fluorescence intensity of CA.Upon the addition of KF,the fluorescence quenching of CA occurred,the change of the CA fluorescence intensity was in good linear relationship with KF concentration.Based on this,a novel fluorescence quenching platform for the determination of KF was constructed.Under the optimum condition,the determination range of KF ranged from 1.0×10-8 mol/L to 3.4×10-6 mol/L with a detection limit of 3.33×10-9 mol/L.The method was applied in the determination of KF in compound honeysuckle particles,with recoveries of 98.4%-103.5% and relative standard deviations（RSD） not more than 2.7%. |
| Key Words:carbon dots calcein fluorescence resonance energy transfer kaempferol |
| 引用本文：陶慧林，廖秀芬，周素莲，钟福新，易忠胜，陈俊.碳点-钙黄绿素荧光共振能量转移体系在山奈素检测中的应用研究[J].分析测试学报,2014,33(7):820-824. |
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