| 超临界色谱法测定化妆品中生育酚及α-生育酚乙酸酯 |
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| 基金项目:国家质检总局科技计划项目（2012QK090）；广东省质量技术监督局科技项目（2013ZS03） |
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| 中文摘要:建立了化妆品中4种生育酚异构体及α-生育酚乙酸酯的超临界色谱分析方法。不同基质类型的化妆品经乙腈水溶液-正己烷溶剂体系按不同的添加顺序进行分散、提取、液液分配后，以二氧化碳为流动相，异丙醇-正己烷(1∶1)为改性剂，利用亚乙基桥杂化颗粒色谱柱(Viridis@BEH,250 mm×4.6 mm,5 μm)对目标物和样品杂质进行有效分离，用光电二极管矩阵检测器(SFC-PDA)进行测定。系统考察了提取条件和色谱条件对实验结果的影响。结果表明：4种生育酚和α-生育酚乙酸酯的定量下限(S/N=10)分别为50 mg/kg和25 mg/kg，空白基质加标回收率为81.3%～109.5%，相对标准偏差(RSD)不大于12.8%。该方法前处理简单、快速、准确，可用于各类化妆品中4种生育酚异构体及α-生育酚乙酸酯含量的检测。 |
| 中文关键词:超临界色谱法 化妆品 生育酚 α-生育酚乙酸酯 |
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| Determination of Tocopherols and α-Tocopheryl Acetate in Cosmetics by Supercritical Fluid Chromatography |
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| Abstract:A supercritical fluid chromatographic(SFC) method was developed for the determination of tocopherols and α-tocopheryl acetate in cosmetics.Different types of samples were sequentialy dispersed and extracted with acetonitrile water solution-hexane based on matix types.The chromatographic separation was conducted with a XBridge(Viridis@BEH,250 mm×4.6 mm,5 μm) by gradient elution using CO2 as mobile phase and 2-propanol∶hexane(1∶1) as modifier.The detection was performed with a photodiode-array(PDA) detector.The extraction procedures and chromatographic conditions were optimized.The results showed that the limits of quantitation(LOQ,S/N=10) were 50 mg/kg for four tocopherols and 25 mg/kg for α-tocopheryl acetate.The average recoveries ranged from 81.3% to 109.5% with relative standard deviations(RSDs) not more than 12.8%.The method is simple,rapid and accurate,and is suitable for the determination of four tocopherols and α-tocopheryl acetate in cosmetics. |
| Key Words:supercritical fluid chromatography(SFC) cosmetics tocopherol α-tocopheryl acetate |
| 引用本文：李慧勇，席绍峰，谭建华，王继才，熊小婷，赵田甜，吴楚森，郭长虹.超临界色谱法测定化妆品中生育酚及α-生育酚乙酸酯[J].分析测试学报,2014,33(4):416-421. |
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