| 采用新型固相萃取柱快速测定食用植物油中苯并［a］芘 |
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| 基金项目:陕西省农业攻关项目(2010K01-19-4)；国家质检总局科研项目(2009IK285)；陕西出入境检验检疫局科研项目(2010IK001) |
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| 中文摘要:研究了Bond Elut ENV新型固相萃取柱在食用植物油中苯并［a］芘快速检测中的应用，建立了快速测定食用植物油样品中苯并［a］芘残留量的固相萃取/液相色谱/荧光检测法。样品用正己烷溶解，固相萃取净化,SUPELCOSILTM LC-PAH(25 cm×4.6 mm，5 μm)色谱柱分离，以乙腈-水(95∶5)为流动相，荧光检测(λex=297 nm，λem=408 nm)，外标法定量。苯并［a］芘的检出限为0.3 μg/kg，在1.0 ~50.0 μg/L范围内线性关系良好，相关系数为0.999 6，方法的回收率为79% ~102%，相对标准偏差不高于9.4%。该方法准确、实用、简便、快速，在食用植物油的苯并［a］芘残留量检测方面有广泛的应用前景。 |
| 中文关键词:Bond Elut ENV固相萃取柱 食用植物油 苯并［a］芘 硅胶固相萃取柱 液相色谱/荧光检测 |
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| Rapid Determination of Benzo［a］pyrene in Edible Vegetable Oil Using a New Type of Solid Phase Extraction Column |
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| Abstract:A new type of solid phase extraction column(Bond Elut ENV)was used for the rapid determination of benzo［a］pyrene in edible vegetable oil.And a solid phase extraction purification and high performance liquid chromatography/fluorescence detection method was developed for the determination of benzo［a］pyrene in edible vegetable oil.Samples were dissolved in n-hexane and then purified by solid phase extraction.Chromatographic analysis was performed on a SUPELCOSILTM LC-PAH(25 cm×4.6 mm，5 μm) column using acetonitrile-water (95∶5) as mobile phase.Quantitation analysis was carried out by the external standard method using fluorescence detecter at λex=297 nm and λem=408 nm.The calibration curve was linear over the benzo［a］pyrene concentration in the range of 1.0-50.0 μg/L(r=0.999 6) with detection limit of 0.3 μg/kg.The recoveries ranged from 79% to 102% with relative standard deviations no more than 94%.The method is accurate,practical,simple and fast,and has an extensive application prospect in the determination of benzo［a］pyrene residues in edible vegetable oil. |
| Key Words:Bond Elut ENV solid phase extraction column edible vegetable oil benzo［a］pyrene silica gel solid phase extraction column liquid chromatography with fluorescence detection |
| 引用本文：何强，孔祥虹，李建华，赵洁，张莹.采用新型固相萃取柱快速测定食用植物油中苯并［a］芘[J].分析测试学报,2012,31(6):710-714. |
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