色谱

色谱 ›› 2013, Vol. 31 ›› Issue (7): 661-666.DOI: 10.3724/SP.J.1123.2013.05027

• 特别策划:庆祝欧庆瑜先生八十华诞专栏 • 上一篇    下一篇

毛细管电泳-间接紫外检测法测定蜂蜜中的氨基酸

周贤婧1,2, 师彦平1   

  1. 1. 中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室, 甘肃 兰州 730000;
    2. 中国科学院大学, 北京 100039
  • 收稿日期:2013-05-16 出版日期:2013-07-28 发布日期:2013-07-17
  • 通讯作者: 师彦平
  • 基金资助:

    国家自然科学基金项目(21075127).

Determination of amino acids in honey by capillary electrophoresis with indirect ultraviolet detection

ZHOU Xianjing1,2, SHI Yanping1   

  1. 1. Key Laboratory of Chemistry of Northwestern Plant Resources, CAS/Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
    2. University of Chinese Academy of Sciences, Beijing 100039, China
  • Received:2013-05-16 Online:2013-07-28 Published:2013-07-17

PDF

211

被引次数

15

摘要：

采用毛细管电泳-间接紫外检测法同时分离测定蜂蜜中的赖氨酸、色氨酸、谷氨酸等9种氨基酸。考察了磷酸浓度、进样方式和缓冲液pH对分离效率和重现性的影响。在分离电压为-15 kV、检测波长为220 nm条件下,以含有0.5 mmol/L十六烷基三甲基溴化铵、20 mmol/L烟酸、10%甲醇的10 mmol/L磷酸二氢钠缓冲溶液(pH 10.2)为运行缓冲液,9种组分在11 min内达到基线分离;检出限最低可达到0.3 mg/L;线性范围为1.0~1000 mg/L;日间及日内精密度为0.64%~5.83%。实际样品中除甲硫氨酸外的8种氨基酸的加标回收率为60.00%~118.37%。将该方法应用于不同蜜源植物和产地的蜂蜜样品的测定,在市售的5种蜂蜜中均检测到脯氨酸、丝氨酸和天冬氨酸,而只在荔枝蜜中检测到苏氨酸。该方法可以为蜂蜜的蜜源鉴别及质量评估提供借鉴方法。

关键词: 氨基酸, 蜂蜜, 间接紫外检测, 毛细管电泳

Abstract:

A method of capillary electrophoresis with indirect ultraviolet (UV) detection was developed for the separation and determination of nine amino acids such as lysine, tryptophan, glutamic acid, etc. The effects of sodium dihydrogen phosphates concentration, pH of buffer and sample injection type and time on the reproducibility and efficiency were investigated. The optimum injection time was 5 s at 5 kPa. The optimum electrophoretic conditions were as follow: 10 mmol/L sodium dihydrogen phosphates (pH 10.2) containing 0.5 mmol/L cetrimonium bromide, 20 mmol/L nicotinic acid and 10%(v/v) methanol as running buffer, applied voltage of-15 kV, detection wavelength of 220 nm. The base line separation of the nine amino acids was achieved successfully within 11 min. The lowest detection limit was 0.3 mg/L. All of the nine analytes showed good linearities within 1.0-1000 mg/L. The relative standard deviations of migration time and peak area were 0.64%-5.83%. The recoveries of the eight amino acids spiked in a real sample were between 60.00% and 118.37%. The method was applied in the determination of the amino acids in honey samples from different nectar plants and origins. Prolin, serine and aspartic acid were found in five honey samples, and tryptophan was only found in a litchi honey sample. This method can provide good reference to the evaluation of the quality and nectar origin of honey.

Key words: amino acids, capillary electrophoresis (CE), honey, indirect ultraviolet detection

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