色谱

色谱

• 研究简报 • 上一篇    下一篇

反相高效液相色谱法测定血浆中的单硝酸异山梨酯

何林,余继英,吴正中,孙世明

  

  1. Laboratory of Clinical Pharmacology, Sichuan Provincial People’s Hospital, Chengdu 610072, China
  • 收稿日期:2004-09-20 修回日期:1900-01-01 出版日期:2005-09-30 发布日期:1988-03-25
  • 通讯作者: 何林

Determination of Isosorbide-5-Nitrate in Human Plasma by Reversed-Phase High Performance Liquid chromatography

HE Lin, YU Jiying, WU Zhengzhong, SUN Shiming   

  1. Laboratory of Clinical Pharmacology, Sichuan Provincial People’s Hospital, Chengdu 610072, China
  • Received:2004-09-20 Revised:1900-01-01 Online:2005-09-30 Published:1988-03-25

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摘要：

建立了反相高效液相色谱测定血浆中单硝酸异山梨酯浓度的方法。样品在碱性条件下经二氯甲烷提取后,用C18柱进行分离，以H2O(用0.03 mol/L氨水调pH至7.8)-乙腈(体积比为80∶20)为流动相,对乙酰氨基酚为内标,于230 nm处测定。结果表明,单硝酸异山梨酯的质量浓度为20~1000 μg/L时其峰面积与内标峰面积之比与单硝酸异山梨酯浓度有良好的线性关系。方法的最低检测质量浓度为12 μg/L,平均回收率为(97.11±2.45)％~(104.34±2.17)%,日内测定的相对标准偏差(RSD)≤2.52%,日间测定的RSD≤5.21%。

关键词: 单硝酸异山梨酯, 反相高效液相色谱法, 血浆

Abstract:

The method was established to determine the level of isosorbide-5-nitrate in human plasma by reversed-phase high performance liquid chromatography (RP-HPLC). The sample preparation was carried by alkalizing the plasma sample followed by extraction with dichloromethane. The HPLC analysis was performed under the conditions as follows: a mixture of an aqueous buffer (pH adjusted to 7.8 by 0.03 mol/L ammonia water) and acetonitrile (80∶20, v/v) as mobile phase, paracetamol as the internal standard, and the detection at 230 nm. The linear range was 20-1000 μg/L using the ratio of peak areas; the detection limit was 12 μg/L; the average recovery was (97.11±2.45)%-（104.34±2.17）%， the intra-day relative standard deviations (RSDs) were less than 2.52%, and inter-day RSDs were less than 5.21%.

Key words: isosorbide-5-nitrate, plasma , reversed-phase high performance liquid chromatography